Effects of Shiga toxin 2 on isolated porcine granulocytes
نویسنده
چکیده
iv CHAPTER 1: INTRODUCTION 1 General Introduction 1 Objectives and Specific Aims 2 CHAPTER 2: LITERATURE REVIEW 6 CHAPTER 3: MATERIALS AND METHODS 15 Stx2 and rStxB2 15 Isolation of Porcine Granulocytes 16 Flow Cytometric Evaluation of Stx2 and rStxB2 Binding and Gb3/CD77 Expression by Porcine Granulocytes 17 Stx2 Binding to Porcine Granulocytes at 4°C 17 Flow Cytometric Analysis 19 Fluorescence Microscopic Evaluation of Stx2 Binding 19 Flow Cytometric rStxB2 Binding Assays 20 Flow Cytometric Gb3/CD77 Receptor Assay 21 Stx2 and rStxB2 Binding to Porcine Granulocytes Under Conditions Required for Functional Studies 21 Internalization of Stx2 and rStxB2 22 Effect of Stx2 on Granulocyte Apoptosis 23 Effect of Stx2 on Granulocyte Oxidative Burst 24 Statistical Analysis 25
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